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Home / Assay Kits

Coenzyme A Assay Kit

SKU : BHE16300246

This product includes 750 ul 10 x CAK reagent. It is for measurement of 1000 samples using 384-well plates. 96-Well plates and cuvettes may also be used for measurements.

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Mfr.No.	CAK1000
Description	The Coenzyme A Assay Kit is for measurement of micromolar concentrations of Coenzyme A (CoA). The assay is based on the principle that the thiol group of CoA interacts with the CAK reagent and enhances the fluorescence intensity at 535 nm (excitation at 485 nm). Acetyl-CoA does not interact with the CAK reagent. The assay reagent is sufficient for measurement of 1000 samples using 384-well plates. The assay kit can be used for measurement of other thiols. The assay is compatible with HEPES buffer, low concentrations of non-ionic detergent (<0.01%), sodium and potassium salt, MgCl2 (< 5 mM), CaCl2 (<5 mM) and phosphate (< 1 mM). The assay is not compatible with ammonium, Tris buffer or other primary amine buffers. It is not compatible with amino acids, DTT or EDTA. The Coenzyme A Assay Kit (catalog number CAK1000) includes 750 µl 10 x CAK reagent. It is for 1000 assays using 384-well plates or 250 assays using 96-well plates. Cuvettes may also be used for measurements.
Concentration measurement	Biomolecules

Product Manual	Datasheet
Protocols Description	PROTOCOL CoA Standard Curve 1. Prepare standard CoA solutions with a series of concentrations from 1 to 200 µM in 10 mM HEPES, pH7.5. 2. Prepare 1 x CAK reagent by dilution of the 10 x CAK reagent with water (10-fold dilution). 3. Mix 120 µl of the CoA solution with 30 µl of 1 x CAK reagent in the wells of a 96-well plate for 10 min and read the fluorescence at 535 nm (excitation at 485 nm). Data Analysis Plot the fluorescence intensity Fc and the CoA concentration [CoA] to generate the linear standard curve. Fc = a [CoA] + b Where the Fc values are from experimental data, the a and b values are from the linear fitting between the Fc values and the CoA concentrations. UNKNOWN SAMPLES Follow the same procedure to measure the fluorescence intensity Fc values from the unknown samples. Calculate the CoA concentrations in the unknown samples using the Fc values from the unknown samples and the a and b values from the standard curve. [CoA] = (Fc – b) / a

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300246	CAK1000	1000 assays	$423.04		Add to Cart

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