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Home / Assay Kits

Beta Octyl Glucoside (ß-OG) Assay Kit

SKU : BHE16300265

This product includes 10 ml of Reagent 1, 5 ml of dilution buffer and 0.1 ml of 100 x Dye for OG. It is for measurement of 100 samples using 96-well plates.

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Mfr.No.	OG100K
Description	The Beta Octyl Glucoside (β-OG) Assay Kit is for measurement of detergent β-OG at concentrations below its CMC value. The assay is based on the principle that β-OG interacts with the dye and enhances the light absorbance at 580 nm. It is compatible with most buffers and salts. It is not compatible with phosphate. Molecules such as ATP or ADP that release phosphate may interfere with the assay. The assay does not detect beta nonyl glucoside (β-NG) or SDS. Please use Beta Nonyl Glucoside (β-NG) Assay Kit (Catalog # NG100K) for measurement of β-NG concentrations; NanoGram SDS Assay Kit (Catalog # SDS200) for measurement of SDS concentrations; and Detergent Assay Kit (Catalog # DAK1000) for other detergents. The assay kit includes 10 ml of Reagent 1, 5 ml of dilution buffer and 0.1 ml of 100 x Dye for OG. It is for measurement of 100 samples using 96-well plates. A cuvette may also be used for the measurement of detergent concentrations.
Concentration measurement	Detergent and lipids

Product Manual	Datasheet
Protocols Description	ASSAY PROTOCOL The following protocol is for assays using a standard black 96-well plate (Costar 3915 or Greiner 655076). Adjust the assay reagent volumes proportionally for measurements using cuvettes. 1. Sample preparation: Prepare 50 µl of standard β-OG solutions with a series of concentrations from zero to 0.2% in 10 mM Tris-HCl, pH 7.5, 100 mM NaCl. Prepare 1 x dye by dilution of the 100 x dye with Reagent 1 (100-fold dilution). Each assay needs 100 µl of 1 x dye. 2. Detection: Mix 50 µl of the detergent solution with 100 µl of 1 x dye and incubate the solution at room temperature for 5 min. Read the light absorbance at 580 nm (A₅₈₀). 3. Data Analysis: Plot the A580 values and the β-OG concentration [β-OG] to generate the linear standard curve. A₅₈₀ = a [β-OG] + b Where the A₅₈₀ values are from experimental data, the a and b values are from the linear fitting between the A₅₈₀ values and the β-OG concentrations. UNKNOWN SAMPLES Follow the same procedure to measure the light absorbance at 580 nm (A₅₈₀) values from the unknown samples. Calculate the β-OG concentrations in the unknown samples using the A₅₈₀ values from the unknown samples and the a and b values from the standard curve. [β-OG] = (A₅₈₀ – β) / a

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300265	OG100K	100 assays	$315.03		Add to Cart

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