The optimal samplingvolume are different for different species, also are different fordifferent sample type. It is recommended to take 2~3 samples to do a pre-experiment,diluting a series of diluent and determine the dilution factor when the inhibition ratio is 10%~50% ( the optimal inhibition ratio is the range of 25%~45%. ) before formal experiment.The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor, Human serum Mouse serum 3-5, Rat serum 5-8, 10% Mouse brain tissue homogenate 10% Rat liver tissue homogenate 30-60, HepG2 cell homogenate (5 mgprot/mL) 10% Plant tissue homogenate 1-5. Note: Thediluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Storage
2-8°C
Valid Period
3 Months
Reviews of Acetylcholinesterase (A-CHE) Activity Assay Kit