96-Well S. aureus gyrase DNA Decatenation Assay Kit Plus (enzyme included)

Mfr.No.	T2DD-96KS
Description	Antibacterial Drug Target. Human DNA topoisomerase II and bacterial DNA topoisomerase IV (the parC-parE complex) converts the concatenated DNA into decatenated DNA (decatenation reaction). Bacterial topoisomerase I and gyrases also have DNA decatenation activity. The 96-Well Topoisomerase DNA Decatenation Assays are in a 96-well assay plate format that can be used for high-throughput tests of human topo II and bacterial topo IV inhibitors. The assay is based on the principle that the decatenated DNA is separated from the concatenated DNA by a filtration process. The decatenated DNA passed through the filter (TDD filter plate), received in a 96 well plate and quantified by fluorescence at 535 nm (excitation at 485 nm). This product includes 600 ul of 10 x assay buffer, 500 ul of 10 x concatenated DNA, 120 ul of 10 mM ATP, 100 ul of 50 x S. aureus gyrase, 600 ul of Stop solution (0.4 M EDTA), 500 ul of 10 x fluorescence dye, 2 ml of 10 x rinse buffer, one V-bottom plate, a TDD filter plate and one black 96-well plates for 96 assays of DNA decatenation reactions.
Drug target assays	Antibacterial drug targets

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300095	T2DD-96KS	96 assays	$894.19		Add to Cart