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Home / Primary Antibodies / Polyclonal Antibodies

ZAP70 antibody

SKU : BHA10809553

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Mfr.No.	FNab09582
Alternative Names	70kDazetaassociatedprotein ; FLJ17670 ; FLJ17679 ; SRK ; STD ; Sykrelatedtyrosinekinase ; TyrosineproteinkinaseZAP70 ; TZK ; ZAP70 ; ZAP70
Background	This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Categories	Primary Antibodies
Cellular Localization	Cytoplasm, Membrane
Clonality	polyclonal
Description	Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).
Host	Rabbit
Immunogen	zeta-chain (TCR) associated protein kinase 70kDa
Isotype	IgG
Molecular Weight	69 kDa
Reactivity	Human, Mouse
Regulatory	RUO
Subcellular Location	Cell membrane, Cytoplasm, Membrane
Synonyms	70 kDa zeta associated protein, FLJ17670, FLJ17679, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

Uniprot	P43403
Gene Id	7535
Research Area	Developmental biology, Immunology, Signal Transduction, Metabolism
Weight	70kDa

Form	liquid
Format	liquid
Purification	Immunogen affinity purified
Purity	>=95% as determined by SDS-PAGE
Storage	PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Applications

ELISA, WB, IHC, IP

Description

ELISA ; WB ; IHC ; IP

Dilution

WB	1:500-1:5000
IHC	1:20-1:200
IP	1:500-1:5000

Tested Application

WB: 1:500 - 1:2000; IHC: 1:50 - 1:200

Manual	FNab09582.pdf

No Description Available.

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SKU	Supplier No.	Size	Your price	Quantity
BHA10809553	FNab09582	100ug	$260		Add to Cart

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