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Home / Primary Antibodies / Polyclonal Antibodies

UBE2T/HSPC150 antibody

SKU : BHA10809176

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Mfr.No.	FNab09185
Alternative Names	HSPC150 ; PIG50 ; UBE2T ; UbiquitincarrierproteinT ; UbiquitinproteinligaseT
Background	The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.
Categories	Primary Antibodies
Cellular Localization	Nucleus
Clonality	polyclonal
Description	The ubiquitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.
Host	Rabbit
Immunogen	ubiquitin-conjugating enzyme E2T(putative)
Isotype	IgG
Molecular Weight	24 kDa
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Subcellular Location	Nucleus
Synonyms	HSPC150, PIG50, UBE2T, Ubiquitin carrier protein T, Ubiquitin protein ligase T

Uniprot	Q9NPD8
Gene Id	28089
Research Area	Epigenetics, Metabolism
Weight	23kDa

Form	liquid
Format	liquid
Purification	Immunogen affinity purified
Purity	>=95% as determined by SDS-PAGE
Storage	PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)

Applications

ELISA, WB, IF, IP

Description

ELISA ; WB ; IF ; IP

Dilution

WB	1:200-1:2000
IP	1:200-1:2000
IF	1:50-1:500

Tested Application

WB: 1:200-1:2000; IP: 1:200-1:2000

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SKU	Supplier No.	Size	Your price	Quantity
BHA10809176	FNab09185	100ug	$260		Add to Cart

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