General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex(PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC(preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
Clonality
polyclonal
Host
Rabbit
Immunogen
TATA box binding protein
Immunogen Region
TATA boX binding protein
Isotype
IgG
Molecular Weight
37-43kDa
Reactivity
Human
Recommended Dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Synonyms
GTF2D1, TF2D, TFIID
Uniprot
P20226
Gene Id
6908
Research Area
Developmental biology, Metabolism, Neuroscience
Form
liquid
Purification
Immunogen affinity purified
Purity
>=95% as determined by SDS-PAGE
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)
Tested Application
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Product Manual
<a href="https://www.fn-test.com/content/uploads/product/manuals/antibody/FNab10263.pdf" target="_blank">Data Sheet</ a>