This recombinant phospho-Stat3 antibody reacts to human Stat3 only when phosphorylated at Tyr705. There is no cross-reactivity to Stat3 without phosphorylation at Tyr705. It may also react to mouse or rat phospho-Stat3 (Tyr705), as predicted by immunogen homology.
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Immunogen Region
A phospho-peptide corresponding to human phospho-Stat3 (Tyr705) was used as the immunogen for this recombinant phospho-Stat3 antibody.
Isotype
Rabbit IgG
Species Reactivity
Human
Note
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Stat3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Uniprot
P40763
Format
Purified
Purity
Protein A purified from animal origin-free supernatant
Storage
Store the recombinant phospho-Stat3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).