Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. (RefSeq)
Formulation
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Host
Rabbit
Immunogen Region
A synthetic peptide specific to human Histone H2A.X / H2AFX was used as the immunogen for the H2AFX antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Subcellular Location
Nuclear
Uniprot
P16104
Clone No
BIA-8
Format
Purified
Purification
Affinity purified
Storage
Store the H2AFX antibody at -20°C.
Applications
IHC-P, WB
Dilution
Western blot: 1:500-1:2000,Immunohistochemistry: 1:50-1:200