This recombinant His Tag antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Formulation
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Immunogen Region
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus was used as the immunogen for this recombinant His Tag antibody.
Isotype
Rabbit IgG
Species Reactivity
All Species
Note
The stated application concentrations are suggested starting points. Titration of the recombinant His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Format
Biotin Conjugate
Purity
Protein A purified from animal origin-free supernatant
Storage
Store the recombinant His Tag antibody at -20oC.
Applications
FACS, WB, ICC, IHC
Description
Western Blot: 0.1-0.5ug/ml,Immunocytochemistry: 0.5-2ug/ml,Flow Cytometry: 0.5-2ug/ml,Immunohistochemistry: 0.1-1ug/ml (1),ELISA: 0.01ug/ml-0.5ug/ml