GCLC antibody

GCLC, the catalytic subunit of glutamate-cysteine ligase, is encoded by a gene located on chromosome 1p22-p21. This enzyme functions as part of a heterodimer, consisting of a heavy catalytic subunit and a light regulatory subunit derived from a separate gene. Together, these subunits form the functional enzyme responsible for the first and rate-limiting step in glutathione biosynthesis, catalyzing the reaction between glutamate and cysteine to produce gamma-glutamylcysteine.

The regulatory subunit modulates the activity of the GCS heavy chain, ensuring proper enzymatic function and cellular homeostasis. Deficiency of this enzyme, particularly the gamma-glutamylcysteine synthetase, is associated with hemolytic anemia due to decreased glutathione levels, underscoring its importance in cellular health.
 

Scientific Background

Glutathione synthesis is critical for maintaining cellular redox balance. The gamma-glutamylcysteine ligase enzyme, comprising a catalytic subunit and a light regulatory subunit, plays a pivotal role in this process. The catalytic subunit (GCLC) is the rate-limiting enzyme in this pathway, and mutations or deficiencies in this enzyme can lead to significant physiological impairments, including hemolytic anemia.

Research on GCLC has expanded our understanding of diseases related to oxidative stress, making this antibody a valuable resource for investigations in fields such as oncology, neurodegeneration, and metabolic disorders.

• Target: GCLC (Gamma-Glutamylcysteine Ligase Catalytic Subunit)

• Applications: Suitable for Weste blot and other immunodetection methods.

• Molecular Function: Encodes the catalytic subunit of the enzyme responsible for glutathione synthesis.

• Structure: The enzyme consists of two subunits, with the catalytic subunit being the active enzymatic component.

• Related Conditions: Deficiency linked to anemia due to deficiency of glutathione.

• Research Use: Designed for studies in oxidative stress, cellular metabolism, and antioxidant pathways.