H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.
Clonality
polyclonal
Host
Rabbit
Immunogen
A synthetic phosphorylated peptide around S139 of human H2AX
Immunogen Region
A synthetic phosphorylated peptide around S139 of human H2AX
Isotype
IgG
Molecular Weight
17 kDa
Reactivity
Human, Mouse, Rat
Recommended Dilution
WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:100
Synonyms
gamma H2A.X,gammaH2AX, Phospho-H2AX-S139
Uniprot
P16104
Gene Id
3014
Research Area
Epigenetics
Form
liquid
Purification
Immunogen affinity purified
Purity
>=95% as determined by SDS-PAGE
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Application
WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:100
Product Manual
<a href="https://www.fn-test.com/content/uploads/product/manuals/antibody/FNab10441.pdf" target="_blank">Data Sheet</ a>