The epidermal growth factor receptor (EGFR; ErbB1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB1), HER2/c-neu (ErbB2), Her 3 (ErbB3) and Her 4 (ErbB4). It exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to overexpression or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiform.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Human partial recombinant protein (AA 25-346) was used as the immunogen for this EGFR antibody.
Isotype
IgG
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Gene Id
1956
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the EGFR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IF
Dilution
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml