EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Formulation
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Host
Mouse
Immunogen Region
A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the anti-EpCAM antibody. The epitope has been mapped to the AA 202-209 region.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
The stated application concentrations are suggested starting amounts. Titration of the Desmoglein 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Uniprot
P16422
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the anti-EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P
Description
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.