Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
Clonality
polyclonal
Host
Rabbit
Immunogen
Chromatin assembly factor 1 subunit B
Immunogen Region
Chromatin assembly factor 1 subunit B
Isotype
IgG
Molecular Weight
70 kDa
Reactivity
Human, Mouse, Rat
Recommended Dilution
WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
Synonyms
CHAF1B, CAF-1, CAF-IP60, CAF1
Uniprot
Q13112
Form
liquid
Purification
Immunogen affinity purified
Purity
>=95% as determined by SDS-PAGE
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)
Tested Application
WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
Product Manual
<a href="https://www.fn-test.com/content/uploads/product/manuals/antibody/FNab10505.pdf" target="_blank">Data Sheet</ a>