Mouse Anti-Human CD74 Monoclonal IgG1

Mfr.No.	SMC-269, SMC-269D, SMC-269D-A390, SMC-269D-A488, SMC-269D-A594, SMC-269D-ALP, SMC-269D-APC, SMC-269D-BI, SMC-269D-FITC, SMC-269D-HRP, SMC-269D-PCP, SMC-269D-RPE, SMC-269S
Immunogen Species	Human
Alternative Names	DHLAG Antibody, HLA DR gamma Antibody, HLADG Antibody, p33 Antibody, p35 Antibody, Protein 41 Antibody, CD 74 Antibody, CD74 Antibody, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) Antibody, CD74 antigen Antibody, CD74 molecule Antibody, CD74 molecule, major histocompatibility complex, class II invariant chain Antibody, CLIP Antibody, DHLAG Antibody, Gamma chain of class II antigens Antibody, HG2A_HUMAN Antibody, HLA class II histocompatibility antigen gamma chain Antibody, HLA DR antigens associated invariant chain Antibody, HLA DR gamma Antibody, HLA-DR antigens-associated invariant chain Antibody, HLA-DR-gamma Antibody, HLADG Antibody, HLADR antigens associated invariant chain Antibody Ia antigen associated invariant chain Antibody, Ia antigen-associated invariant chain Antibody, Ia associated invariant chain Antibody, Ia gamma Antibody, Ii Antibody, Invariant polypeptide of major histocompatibility complex class II antigen associated Antibody, la-gamma Antibody, Major histocompatibility complex class II invariant chain Antibody, MHC HLA DR gamma chain Antibody, MHC HLA-DR gamma chain Antibody, p33 Antibody, p35 Antibody, Protein 41 Antibody
Categories	Antibodies
Cellular Localization	Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Cell Membrane, Golgi Apparatus, Endosome, Lysosome
Certificate Of Analysis	A 1:1000 dilution of SMC-269 was sufficient for detection of CD74 in 15 ug of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Clonality	Monoclonal
Description	Mouse Anti-Human CD74 Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide corresponding to the N-terminal (1-100 aa) of human CD74 conjugated to KLH. Overlaps 100% with all 3 isoforms, Homo Sapiens (Human)
Immunogen Region	Synthetic peptide corresponding to the N-terminal (1-100 aa) of human CD74 conjugated to KLH. Overlaps 100% with all 3 isoforms
Isotype	IgG1
Launch Date	2017/03/23
Net Weight (g)	0.1
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Human

Swissprot	P04233
Accession Number	NP_001020329.1
Gene Id	972
Target	CD74
Clone No	6D9
Research Area	Immunology, Adaptive Immunity, B Cell CD Markers, B Cells

Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~33 kDa protein.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Becker-Hermann, S., Arie, G., Medvedovsky H, Kerem A, and Shachar I. (2005) Mol Bio Cell. 16(11):5061-9. 2. Barrera CA., et al (2005) J Histochem Cytochem 53 (12): 1481-9. 3. Starlets D., et al. (2006) Blood. 107 (12): 4807-4816. 4. Burton JD., et al. (2004). Clin Cancer Res. 10(19): 6606-11. 5. Denzin L.K., Hammond, C. and Cresswell, P. (1996) J. Exp. Med. 184: 2153-2165. 6. Denzin L.K., Robbins N.F., Carboy-Newcomb C. and Cresswell P. (1994) Immunity 1: 595-606.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11902424-1	SMC-269D	Unconjugated	100 ug	$330	Add to Cart
BHA11902424-2	SMC-269D-A390	ATTO 390	100 ug	$384	Add to Cart
BHA11902424-3	SMC-269D-A488	ATTO 488	100 ug	$383	Add to Cart
BHA11902424-4	SMC-269D-A594	ATTO 594	100 ug	$383	Add to Cart
BHA11902424-5	SMC-269D-ALP	Alkaline Phosphatase	100 ug	$376	Add to Cart
BHA11902424-6	SMC-269D-APC	APC	100 ug	$382	Add to Cart
BHA11902424-7	SMC-269D-BI	Biotin	100 ug	$378	Add to Cart
BHA11902424-8	SMC-269D-FITC	FITC	100 ug	$374	Add to Cart
BHA11902424-9	SMC-269D-HRP	HRP	100 ug	$370	Add to Cart
BHA11902424-10	SMC-269D-PCP	PerCP	100 ug	$382	Add to Cart
BHA11902424-11	SMC-269D-RPE	RPE	100 ug	$380	Add to Cart
BHA11902424-12	SMC-269S	Unconjugated	12 ug	$44	Add to Cart

CD74 Antibody, Clone 6D9 - SMC-269

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