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Home / Antibodies

CD44 Antibody / HCAM (V8249)

SKU : BHA17117774

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NSJ Bioreagents

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Mfr.No.	V8249, V8249-20UG, V8249-100UG
Clonality	Monoclonal
Description	Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Formulation	0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host	Mouse
Immunogen Region	Follicular dendritic cells and germinal B cells were used as the immunogen for this CD44 antibody.
Isotype	IgG2a,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No
Subcellular Location	Cell surface, cytoplasmic

Uniprot	P16070
Clone No	BU75

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	FACS, IF, WB, IHC-P
Dilution	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17117774-1	V8249-20UG	20ug	$219		Add to Cart
BHA17117774-2	V8249-100UG	100ug	$499		Add to Cart

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