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Home / Primary Antibodies / Polyclonal Antibodies

beta actin antibody

SKU : BHA10800864

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Mfr.No.	FNab00869
Alternative Names	ACTB ; Actin ; actin ; beta ; Actin ; cytoplasmic1 ; Bactin ; Betaactin ; Factin ; βactin
Background	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha(ACTC1, cardiac muscle 1), alpha 1(ACTA1, skeletal muscle) and 2(ACTA2, aortic smooth muscle), beta(ACTB), gamma 1(ACTG1) and 2(ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2(rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.
Categories	Primary Antibodies
Clonality	polyclonal
Description	Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.
Host	Rabbit
Immunogen	actin, beta
Isotype	IgG
Molecular Weight	42 kDa
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Synonyms	ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, beta actin

Uniprot	P60709
Research Area	Cell Division and Proliferation, Cardiovascular, Metabolism
Weight	42kDa

Form	liquid
Format	liquid
Purification	Immunogen affinity purified
Purity	>=95% as determined by SDS-PAGE
Storage	PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)

Applications

ELISA, IF, WB, IHC

Description

ELISA ; IF ; WB ; IHC

Dilution

WB	1:1000-1:10000
IHC	1:20-1:500
IF	1:10-1:100

Tested Application

WB: 1:500-1:5000; IHC: 1:20-1:500; IF: 1:50-1:500;IP: 1:500-1:2000

Manual	FNab00869.pdf

No Description Available.

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SKU	Supplier No.	Size	Your price	Quantity
BHA10800864	FNab00869	100ug	$240		Add to Cart

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