Mitochondrial membrane ATP synthase(F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1)-containing the extramembraneous catalytic core, and F(0)-containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Clonality
polyclonal
Host
Rabbit
Immunogen
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
Immunogen Region
ATP synthase, H+ transporting, mitochondrial F0 compleX, subunit d
Isotype
IgG
Molecular Weight
19-22 kDa
Reactivity
Human, Mouse
Recommended Dilution
WB: 1:500-1:2000; IHC: 1:50-1:500
Synonyms
ATP5H, ATP5JD, ATPase subunit d, ATPQ
Uniprot
O75947
Gene Id
10476
Research Area
Signal Transduction, Metabolism
Form
liquid
Purification
Immunogen affinity purified
Purity
>=95% as determined by SDS-PAGE
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)
Tested Application
WB: 1:500-1:2000; IHC: 1:50-1:500
Product Manual
<a href="https://www.fn-test.com/content/uploads/product/manuals/antibody/FNab10033.pdf" target="_blank">Data Sheet</ a>