Activating Fragment 1, CAP20, CDK-interacting protein 1, CDKI, CDKN1, CDKN1A, CIP1, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), DNA Synthesis Inhibitor, MDA6, Melanoma Differentiation Associated Protein 6, p21Cip1/Waf1, PIC1, SDI1, SLC12A9, Wild type p53 activated fragment 1 (WAF1)
Categories
Primary Antibodies
Cellular Localization
Nuclear
Chromosome Location
6p21.31
Clonality
monoclonal
Description
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
Host
Mouse
Immunogen
Recombinant full-length human CDKN1A protein
Isotype
IgG1 Kappa
Positive Control
HeLa or MCF7 cells.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Species
Homo Sapiens (Human)
Swissprot
P38936
Gene Id
1026
Clone No
AC8
Symbol
CDKN1A
Weight
21kDa
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Applications
ELISA, WBCLIP, IF
Description
Optimal dilution for a specific application should be determined., ELISA ; WBCLIP ; IF
Dilution
ELISA
For coating use Ab at 1-5μg/ml, order Ab without BSA
Western Blotting
0.5-1μg/ml
Immunoprecipitation
1-2μg/500ug protein
Immunofluorescence
1-2μg/ml
Reviews of Anti-undefined Antibody AC8 Unconjugated BHA10208269