Anti-Orexin Receptor 2 Antibody

Mfr.No.	Y051682
Categories	Primary Antibodies
Clonality	polyclonal
Description	Orexin Receptor 2 (OX2R) (also known as hypocretin receptor 2) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX2R binds both orexin A and B with similar affinities while OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference).2,3Both OX2R and OX1R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. OX2R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels. OX2R can also transmit signals through inhibitory Gi G proteins, although the mechanism is less well understood.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research over the last few years. OX2R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2The best studied physiological role of OX2R is its involvement in the regulation of sleep and wakefulness states. Studies in mice lacking the orexin gene and dogs expressing a null mutation of the OX2R show a remarkably similar phenotype to human narcolepsy, a condition characterized by excessive daytime sleepiness, inability to maintain vigilant states, and defects in the regulation of rapid eye movement (REM) sleep.4In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4We are pleased to offer a highly specific antibody directed against an epitope located in the intracellular C-terminal domain of rat Orexin Receptor 2. Anti-OX2R antibody can be used in Western blot, immunocytochemical, and immunohistochemical applications and recognizes OX2R from rat, mouse, and human samples.
Formulation	Liquid
Host	Rabbit(Rb)
Guarantee	abm guarantees that all our Anti-Orexin Receptor 2 Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen	Peptide (C)DRLARGRTSTESRKS corresponding to amino acid residues 391-405 of rat OX2R (Accession P56719).
Isotype	IgG
Quantity	50μl
Recommended Dilution	For research purposes only, not for human use.
Shipping Condition	Ice Packs
Species Reactivity	Human ; Mouse ; Rat

Gene Name	Orexin Receptor 2
Clone No	Polyclonal

Concentration	1µg/ul
Purity	Affinity purified on immobilized antigen.
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition	-20C