This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.05-0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Formulation
Liquid
Host
Mouse(Mu)
Guarantee
abm guarantees that all our Anti-human IgE Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Immunogen
Myelomatous human serum
Isotype
IgG, IgG1, IgG2a
Quantity
100 μg
Recommended Dilution
Detection of total IgE level and IgE antibodies in ELISA.Recommended pairs (coating - conjugate): 5D4 – 4F4, 4G7– BE5.MAb E411 can be used for coating.MAb 4F4 can be used for IgE purification by affinity chromatography.MAb XTE4 can be used in Western blotting.
Shipping Condition
Ice Packs
Species Reactivity
Human
Specificity
MAb E411 class specific for human IgE, Fc-region specificMAb 4F4 - IgE Cε3-domain specificMAb 5D4 - IgE Cε2-domain specificMAb XTE4 - epsilon chain specificSpecific antibody titer is 1/1 000 000 by indirect ELISA.
Gene Name
IgE
Clone No
Monoclonal
Concentration
1µg/ul
Purity
MAbs 4F4, 5D4, E411, BE5, 4G7 and 4H10 by chromatography on protein A SepharoseMAb XTE4 by ion exchange chromatography
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.