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Home / Primary Antibodies

Anti-GAPDH Polyclonal Loading Control Antibody

SKU : BHA10901472

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Y058203
Categories	Primary Antibodies ; Control Antibodies
Cellular Localization	Cytoplasmic
Clonality	polyclonal
Description	This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alteative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Host	Rabbit
Immunogen	Synthetic peptide conjugated to KLH
Isotype	IgG
Quantity	100 µl
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO
Shipping Condition	Ice Packs

Antigen	GAPDH

Buffer	PBS, pH 7.4 with 0.02% Sodium Azide
Concentration	100µg/100ul
Description	Specificity: Not yet tested in other species. Relevance: Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified.
Format	Liquid
Purity	Affinity purified
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition	-20C

Applications

ELISA

Description

For research use onlyPeptide ELISA titer: 1:20,000-1:80,000 Weste blot analysis: 1:500-1000 Optimal dilutions/concentrations should be determined by the end use., ELISA

Dilution

ELISA	1:20,000-1:80,000
WB	1:500-1000

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SKU	Supplier No.	Size	Your price	Quantity
BHA10901472	Y058203	100 ul	$232.50		Add to Cart

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