Anti-Complement 4d (C4d) Antibody BHA10205513

Mfr.No.	720-MSM1X-P0
Alternative Names	Acidic Complement C4 ; Basic C4 ; C4 ; C4-1 ; C4a Anaphylatoxin ; C4A ; C4A2 ; C4A3 ; C4A4 ; C4A6
Categories	Primary Antibodies
Cellular Localization	Intracytoplasmic vacuoles of endothelial cells(secreted)
Chromosome Location	6p21.3
Clonality	monoclonal
Description	This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.ĀC4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.
Host	Mouse
Immunogen	Recombinant human Complement 4d protein
Positive Control	Rejected Renal Transplant Tissue
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P0C0L4; P0C0L5
Uniprot	534847; 720022
Gene Id	720;721

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

ELISA, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., ELISA ; IF ; IHC-F

Dilution

ELISA	For coating use Ab at 1-2μg/ml,order Ab without BSA
IF	1:50-1:100
IHC-F	1:200-1:400 for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10205513	720-MSM1X-P0	20µg	$204.67		Add to Cart
BHA10205513	720-MSM1X-P0	100µg	$393.20		Add to Cart

Anti-Complement 4d (C4d) Antibody BHA10205513

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