This anti-CD123 antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested in flow cytometry assay for specific binding to human CD123 on the surface of CD123 CHO Recombinant Cell Line (BPS Bioscience #79640).
assay conditions: One million CD123 CHO recombinant cells (BPS Bioscience #79640) were blocked and stained with 1 ug of anti-CD123 antibody, PE-labeled (BPS Bioscience #101674) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CD123 CHO recombinant cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.
Formulation
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain protein stabilizer.
Host
HEK293
Isotype
IgG1
Species
Human
Synonyms
Cell division cycle protein 123 homolog, Protein D123, HT-1080, PZ32, CDC123, C10orf7, D123
Note
Do not freeze
Uniprot
O75794
Concentration
The concentration will vary with each lot; the exact value will be provided with each shipment.
Format
Aqueous buffer solution
Purification
Protein A affinity chromatography from HEK293 supernatants
Purity
>=90%
Shipping Temperature
+4°C (gel packs)
Storage Stability
At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.