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Home / Primary Antibodies

Anti-Bcr (Phospho-Tyr177) Antibody

SKU : BHA10917928

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Applied Biological Materials (abm) Inc.

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Mfr.No.	Y011199
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Host	Rabbit
Immunogen	The antiserum was produced against synthesized phosphopeptide derived from human Bcr around the phosphorylation site of tyrosine 177 (P-F-Y-VP-N).
Quantity	100 µg
Reactivity	Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Shipping Condition	Ice Packs

Buffer	phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	1µg/ul
Description	Specificity: Bcr (phospho-Tyr177) antibody detects endogenous levels of Bcr only when phosphorylated at tyrosine 177.
Format	Liquid
Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition	-20C

Applications

WB, IHC

Description

WB: 1:500~1:1000 IHC: 1:50~1:100, WB ; IHC

Dilution

WB	1:500-1:1000~
IHC	1:50-1:100~

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SKU	Supplier No.	Size	Your price	Quantity
BHA10917928	Y011199	100 ug	$427.50		Add to Cart

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