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Home / Primary Antibodies / Polyclonal Antibodies

6*his,His tag antibody

SKU : BHA10800010

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Mfr.No.	FNab00010
Alternative Names	6His ; 6His ; HisTag ; 6histag ; 6XHis ; HHHHHH ; HIS ; HisTag ; HISTIDINETAGGED
Background	Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Categories	Primary Antibodies ; Tag & Cell Markers
Clonality	polyclonal
Description	Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Host	Rabbit
Isotype	IgG
Reactivity	Recombinant Protein
Regulatory	RUO
Synonyms	6His, 6His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag, HISTIDINE TAGGED

Form	liquid
Format	liquid
Purification	Immunogen affinity purified
Purity	>=95% as determined by SDS-PAGE
Storage	PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)

Applications

ELISA, WB

Description

ELISA ; WB

Dilution

WB	1:1000-1:5000

Tested Application

WB: 1:500-1:3000

Manual	FNab00010.pdf

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SKU	Supplier No.	Size	Your price	Quantity
BHA10800010	FNab00010	100ug	$240		Add to Cart

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