Anti-FcGR3A-anti-EGFR bispecific IgG antibody, which recognizes FcGR3A (also known as CD16A) and EGFR antigen binding proteins. The amino acid sequences correspond to the sequences from the bispecific EGFR/FcGR3 antigen-binding protein patent WO2019175368A1. This bispecific antibody has been tested for specific binding affinity to purified human FcGR3A and EGFR recombinant protein.
assay conditions: Functional validation: The antibody was validated by measuring anti-FcGR3A-anti-EGFR binding to FcGR3A and EGFR antigens in ELISA assay. The FcGR3A protein (BPS Bioscience #79013) and EGFR protein were coated onto a 96-well plate overnight at 4°C (50 ul/well at a concentration of 2 ug/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 ul of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 ul/well of purified anti-FcGR3A-anti-EGFR antibody (BPS Bioscience #101535), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host
HEK293
Instructions for use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Molecular Weight
Heavy Chain: 76 kDa; Light Chain: 23 kDa + glycans