Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences. A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex. Binding specificity is based on the gRNA and a three nucleotide NGG sequence called the protospacer adjacent motif (PAM) sequence. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This adenovirus expresses a codon-optimized Cas9 nuclease, along with a mCherry reporter. It can be used along with guided RNA for genome-editing.This adenovirus expresses a FLAG tagged Cas9 with a mCherry reporter.
Categories
Adenovirus, Over-Expression, CRISPR/Cas9
Product Type
Pre-Made
Virus Type
Adenovirus
Function
CRISPR/Cas9
Promoter
CAG (ubiquitous)
Reporter
mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Shelf Life
12 Months
HS Code
3822190000
UNSPSC Code
41106621
Insert Gene
mCherry-FLAG-hCas9
Availability
Ready to Ship
Titer
1x10^10 PFU/ml
Reporter
mCherry
Storage
-80C
Shipping
Dry Ice
Reviews of FLAT tagged CRISPR/Cas9 nuclease Adenovirus (Ad-mCherry-FLAG-hCas9)