FLAG tagged CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus (Ad(RGD)-mCherry-FLAG-hCas9)

Mfr.No.	1907
Description	The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This RGD-fiber modified adenovirus expresses a codon-optimized Cas9 nuclease with N-terminal FLAG tag and a mCherry reporter. It can be used along with guided RNA for genome-editing in cells that regular Ad5 doesn't infect well, including human or mouse macrophages, ES cells, T cells, synoviocytes, certain fibroblast and islet grafts etc.
Categories	Adenovirus, Over-Expression, Fiber-modified Ad5
Product Type	Pre-Made
Virus Type	Adenovirus
Function	CRISPR/Cas9
Promoter	CAG (ubiquitous)
Reporter	mCherry
Storage Buffer	DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone	Human Adenovirus Type5 (dE1/E3) with RGD-fiber modification
Shelf Life	12 Months
HS Code	3822190000
UNSPSC Code	41106621
Insert Gene	mCherry-FLAG-hCas9
Serotype	RGD
Availability	Ready to Ship
Titer	1x10^10 PFU/ml
Reporter	mCherry
Storage	-80C
Shipping	Dry Ice

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SKU	Supplier No.	Size	Your price	Quantity
BHV21600436	1907	200 µL	$690		Add to Cart