codon-optimized FLP (FLPo) with mCherry Adenovirus (Ad-mCherry-FLPo)

Mfr.No.	1778
Description	FLP is a site-specific recombinases (SSR) from the λ integrase family, which recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency in mouse cells. This adenovirus express both FLPo and mCherry as a fluorescent marker. FLPo and mCherry are driven by the same CMV promoter and separated by a 2A peptide.
Categories	Adenovirus, Over-Expression, Recombinase
Product Type	Pre-Made
Virus Type	Adenovirus
Function	Reporter/Tag
Promoter	CMV (ubiquitous)
Reporter	mCherry
Storage Buffer	DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone	Human Adenovirus Type5 (dE1/E3)
Shelf Life	12 Months
HS Code	3822190000
UNSPSC Code	41106621
Insert Gene	mCherry-FLPo
Availability	Ready to Ship
Titer	1x10^10 PFU/ml
Reporter	mCherry
Storage	-80C
Shipping	Dry Ice

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SKU	Supplier No.	Size	Your price	Quantity
BHV21600346	1778	200 µL	$495		Add to Cart