Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals. By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.The AAV-DJ is a synthetic serotype made from DNA family shuffling of 8 wild type serotypes of AAV, including AAV2, 4, 5, 8, 9, avian, bovine and goat AAV. AAV-DJ outperformed standard AAV serotype 1-8 in cultured cells.This AAV-DJ stock expresses an improved Cre (iCre) and a mCherry driven by one CMV promoter. The iCre and mCherry was separated by a 2A peptides.
Categories
AAV, Over-Expression, Cre Recombinases
Product Type
Pre-Made
Virus Type
AAV
Function
Reporter/Tag
Promoter
CMV (ubiquitous)
Reporter
mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV
Shelf Life
12 Months
HS Code
3822190000
UNSPSC Code
41106621
Insert Gene
mCherry-iCre
Serotype
AAV2/DJ
Availability
Ready to Ship
Titer
1x10^13 GC/ml
ITRs
AAV2
Reporter
mCherry
Reviews of AAV-DJ-mCherry-iCre AAV (AAVDJ-mCherry-iCre)