The 6× DNA Loading Buffer is used for the preparation of DNA samples prior to agarose gel electrophoresis or polyacrylamide-agarose gel electrophoresis. The buffer components have been optimized, with the dye solution including indicators Bromophenol Blue and Xylene Cyanol FF, enabling visual monitoring of DNA migration during electrophoresis. Glycerol ensures the sample settles at the bottom of the loading wells, while EDTA chelates divalent metal ions and inhibits metal ion-dependent nucleases.
Components
The 6× DNA Loading Buffer includes the following components: