2x Hieff^TM PCR Master Mix (With Dye)

Mfr.No.

10102ES08, 10102ES03, 10102ES50

Description

2x PCR Master Mix (With Dye) is a 2x PCR master mix with bromophenol blue dye. This 2x PCR master mix from Yeasen contains Hieff^TM Taq DNA Polymerase, dNTPs, and other PCR-required components, which are optimized for conventional PCR. This Taq polymerase master mix provided by Yeasen is characterized by adding pre-loaded bromophenol blue dye, the PCR products using this PCR mix can be loaded directly for electrophoresis after PCR reaction. Moreover, the PCR products contain 3 '-dA protrusion and can be easily cloned into a T vector.

Features

Convenient, ready-to-use mix
Thermostable—half-life is more than 40 min at 95°C
Generates PCR products with 3'-dA overhangs

Specifications

Fidelity (vs. Taq)	1 ×
Hot Start	No
Overhang	3'-A
Polymerase	Taq DNA Polymerase
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Product Type	PCR Master Mix (2×)

Reference	[1] Wu LY, Shang GD, Wang FX, et al. Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration. Dev Cell. 2022;57(4):526-542.e7. doi:10.1016/j.devcel.2021.12.019(IF:12.270) [2] Li Y, Wang C, Zhang L, Chen B, Mo Y, Zhang J. Claudin-5a is essential for the functional formation of both zebrafish blood-brain barrier and blood-cerebrospinal fluid barrier. Fluids Barriers CNS. 2022;19(1):40. Published 2022 Jun 3. doi:10.1186/s12987-022-00337-9(IF:7.662) [3] Luo T, Wang Y, Tang H, et al. An AAV-Based NF-κB-Targeting Gene Therapy (rAAV-DMP-miR533) to Inflammatory Diseases. J Inflamm Res. 2022;15:3447-3466. Published 2022 Jun 14. doi:10.2147/JIR.S362732(IF:6.922) [4] Chen Z, Qi Z, He D, et al. Strategy for Scanning Peptide-Coding Circular RNAs in Colorectal Cancer Based on Bioinformatics Analysis and Experimental Assays. Front Cell Dev Biol. 2022;9:815895. Published 2022 Feb 25. doi:10.3389/fcell.2021.815895(IF:6.684) [5] Yu Y, Fang L. CircRPAP2 regulates the alteative splicing of PTK2 by binding to SRSF1 in breast cancer. Cell Death Discov. 2022;8(1):152. Published 2022 Apr 2. doi:10.1038/s41420-022-00965-y(IF:5.241) [6] Zhang J, Liu W, Li G, et al. BCAS2 is involved in alteative splicing and mouse oocyte development. FASEB J. 2022;36(2):e22128. doi:10.1096/fj.202101279R(IF:5.192) [7] Xiao W, Li J, Zhang Y, et al. A fungal Bipolaris bicolor strain as a potential bioherbicide for goosegrass (Eleusine indica) control. Pest Manag Sci. 2022;78(3):1251-1264. doi:10.1002/ps.6742(IF:4.845) [8] Zhang Y, Yu R, Tang J, et al. Three cytochrome P450 CYP4 family genes regulated by the CncC signaling pathway mediate phytochemical susceptibility in the red flour beetle, Tribolium castaneum. Pest Manag Sci. 2022;78(8):3508-3518. doi:10.1002/ps.6991(IF:4.845) [9] Zhang X, Yang S, Chen W, et al. Circular RNA circYPEL2: A Novel Biomarker in Cervical Cancer. Genes (Basel). 2021;13(1):38. Published 2021 Dec 23. doi:10.3390/genes13010038(IF:4.096) [10] Gu K, Qian D, Qin H, et al. A novel mutation in KCNH2 yields loss-of-function of hERG potassium channel in long QT syndrome 2. Pflugers Arch. 2021;473(2):219-229. doi:10.1007/s00424-021-02518-1(IF:3.657) [11] Yang Y, Chu X, Nie M, et al. A novel long-range deletion spanning STX16 and NPEPL1 causing imprinting defects of the GNAS locus discovered in a patient with autosomal-dominant pseudohypoparathyroidism type 1B. Endocrine. 2020;69(1):212-219. doi:10.1007/s12020-020-02304-6(IF:3.235) [12] Ullah H, Arbab S, Khan MIU, et al. Circulating cell-free mitochondrial DNA fragment: A possible marker for early detection of Schistosoma japonicum. Infect Genet Evol. 2021;88:104683. doi:10.1016/j.meegid.2020.104683(IF:2.773) [13] Xu L, Chen Y, Shen T, Lin C, Zhang B. Genetic Analysis of PICK1 Gene in Alzheimer's Disease: A Study for Finding a New Gene Target. Front Neurol. 2019;9:1169. Published 2019 Jan 9. doi:10.3389/fneur.2018.01169(IF:2.635) [14] Ullah H, Qadeer A, Giri BR. Detection of circulating cell-free DNA to diagnose Schistosoma japonicum infection. Acta Trop. 2020;211:105604. doi:10.1016/j.actatropica.2020.105604(IF:2.555) [15] Yan ZC, Hua HQ, Qi GY, Li YX. Early Detection and Identification of Parasitoid Wasps Trichogramma Westwood (Hymenoptera: Trichogrammatidae) in Their Host Eggs Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism [published online ahead of print, 2022 Jun 24]. J Econ Entomol. 2022;toac095. doi:10.1093/jee/toac095(IF:2.381) [16] Zhou Z, Yin H, Suye S, Zhu F, Cai H, Fu C. The changes of DNA double-strand breaks and DNA repair during ovarian reserve formation in mice. Reprod Biol. 2022;22(1):100603. doi:10.1016/j.repbio.2022.100603(IF:2.376)

Storage	The 2×Hieff™ PCR Master Mix products should be stored at -15℃ ~ -25℃ for 2 years.

Manual	Manual Safety Data Sheet

The expected 1.2 kb PCR products can be amplified with 2×Hieff PCR Master Mix. The Master Mix was stored at -20°C for 1 year following another 3 months at 4°C and 1 month at 25°C. Template: Arabidopsis genome. Annealing temperature: 60°C. Extension time: 40 sec.

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Combination SKU	Supplier No.	Size	Your price
BHR20800004-2	10102ES03	1mL 100T	$15	Add to Cart
BHR20800004-1	10102ES08	5x 1mL 500T	$65	Add to Cart
BHR20800004-3	10102ES50	50x1mL 5000T	$490	Add to Cart

2x Hieff^TM PCR Master Mix (With Dye)

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